The phenomenon of emission of radiation when the electronic state of a molecule falls from an excited to a relaxed state is called fluorescence.
Fluorimetry is the technique that measures quantitatively the intensity of the emitting fluorescence that occurs in nanoseconds at a specific wavelength. The values of the wavelengths which fluorescent molecules get the excited state and, consecutively the maximum of emission in form of energy, are characteristic of fluorophores. This property can be used to identify the presence of different compounds in mixtures, determination of concentration, photobleaching, quenching, HOMO and LUMO energies.
Scientific Reports, 2016, 6: 22918.
Nanoquim Platform is equipped with Fluorescence Spectrometers LS-45 from Perkin-Elmer and can operate the following measurements:
The equipment can work with liquid and solid samples. Set ranges for emission and for excitation are 200-900 nm and 200-800 nm, respectively and the slits are fixed to 10 nm. Attenuators of the signal of 1% and 4% are also available for very emitting samples.
We encourage you to book an experiment with it!
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